Vector Biology Question Paper focuses on “Single Stranded DNA Vectors Cloning – 2”.
1. Single-stranded DNA was used in __________
A. Dideoxy method
B. Replication
A. Translational
D. Hybridization
Answer: A
Clarification: Sequencing by the original dideoxy method required single stranded DNA, as do techniques for oligonucleotide directed mutagenesis.
2. Single stranded form combines cloning, amplification and __________________ of and originally double-stranded DNA fragment.
A. Strand separation
B. Replication
A. Transcription
D. Translation
Answer: A
Clarification: Single-stranded form is an attractive means of combining the cloning, amplification and strand separation of an originally double-stranded DNA fragment.
3. RF acts just like a ___________
A. Phage
B. Host
A. Plasmid
D. Hybrid
Answer: C
Clarification: The phage DNA is replicated via a double stranded circular intermediate. This replicative form can be purified and manipulated in vitro just like a plasmid.
4. RF and single-stranded DNA __________ the competent E.coli cells.
A. Infect
B. Transfect
A. Kill
D. Translate
Answer: B
Clarification: Both RF and single stranded DNA will transfect competent E.coli cells to yield either plaques or infected colonies, depending on the assay method.
5. There are no packaging constraints in single-stranded vectors.
A. True
B. False
Answer: A
Clarification: The size of the phage particle is governed by the size of the viral DNA and therefore there are no packaging constraints associated.
6. Agarose gel electrophoresis is used to determine the _________ of the insert.
A. Activity
B. Size
A. Orientation
D. Replication
Answer: C
Clarification: If two clones carry the insert in opposite directions, the single stranded DNA from them will hybridize and this can be detected by agarose gel electrophoresis.
7. Filamentous phages do not possess the advantages of plasmids.
A. True
B. False
Answer: B
Clarification: As vectors, filamentous phages possess all the advantages of plasmids while producing particles containing single stranded DNA in an easily obtainable form.
8. Unlike lambda, filamentous phages do not have ____________
A. Codons
B. Nucleases
A. Cloning sites
D. Transcription factors
Answer: C
Clarification: Unlike lambda, the filamentous coliphages do not have any non-essential genes which can be used as cloning sites, but intergenic sequences are present.
9. The M13 intergenic sequence contains ________
A. Origins
B. Termination sequence
A. Replication site
D. Nucleosome
Answer: A
Clarification: In M13 there is a 507 bp intergenic region, from position 5498 to 6005 of the DNA sequence, which contains the origins of DNA replication for both the viral and the complementary strands.
10. The first use of M13 as a cloning vector made use of _______ sites.
A. 4
B. 8
A. 10
D. 12
Answer: C
Clarification: The first example of M13 cloning made use of one of 10 BsuI sites in the genome, two of which are in the intergenic sequence region.
11. Insertion of fragments into lac region destroys __________
A. Blue color
B. White color
A. Transparency
D. Opaqueness
Answer: A
Clarification: Insertion of DNA fragments into the lac region of M13 mp1 destroys its ability to form the blue plaques, making detection easy.
12. The lac region does not contain any restriction site for which of the following?
A. AvaII
B. PvuII
A. EcoR1
D. BglII
Answer: C
Clarification: The lac region only contains unique sites for AvaII, BglII, and PvuI and three sites for PvuII and there are no sites anywhere on the complete genome.
13. Which process was used to create an EcoR1 site in the lac region of coliphage vectors?
A. Hybridization
B. Mutagenesis
A. Replication
D. Restriction
Answer: B
Clarification: Groneborn and Messing (1978) used in vitro mutagenesis to change a single base pair, thereby creating a unique EcoR1 site within.
14. The M13 mp2 contained ________
A. Cloning site
B. Nuclease
A. Codon
D. EcoR1 site
Answer: D
Clarification: Groneborn and Messing (1978) used in vitro mutagenesis to change a single base pair, thereby creating a unique EcoR1 site within the lac fragment, called M13 mp2.
15. The derivatives of M13 vector are exact counterparts of _________
A. Pbr322
B. PUC
A. Lambda
D. Adenovirus
Answer: B
Clarification: The derivatives mp7-11, mp18, mp19 are the exact M13 counterparts of the PUC plasmids, containing many common cloning sites.
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