Gene Manipulation Multiple Choice Questions on “Large Scale Mutagenesis and Interference”.
1. Mutations can be introduced by ____________
A. Cloning
B. Recombination
A. Ligation
D. Gene targeting
Answer: D
Clarification: Mutations can be introduced into predefined genes in vivo through a process termed as gene targeting which involves homologous recombination.
2. Gene-knockout is the generation of a null-gene.
A. True
B. False
Answer: A
Clarification: When the aim is to inactivate the target gene completely and generate a null allele, the term gene knockout is often used.
3. Embryonic stem cells are __________
A. Totipotent
B. Pluripotent
A. Dead
D. Multipotent
Answer: B
Clarification: Embryonic stem cells can be cultured like any established cell line, but they are derived from the very early mouse embryo and are therefore pluripotent.
4. The only organism in which systematic gene targeting has been achieved is ____________
A. Mouse
B. Drosophila
A. Fish
D. Yeast
Answer: D
Clarification: The only organism in which systematic gene targeting has been achieved is the yeast Saccharomyces Cerevisiae. Its genome contains about 6000 open reading frames.
5. The EUROFAN project involves the use of _________ generated cassettes.
A. PCR
B. Ultracentrifugation
A. Electroporation
D. Cloning
Answer: A
Clarification: The EUROFAN project involves the use of PCR-generated cassettes in which a selectable marker is placed between 50 basepair elements.
6. Genome-wide random mutagenesis is applicable to _________
A. Fish
B. Mammals
A. All organisms
D. Bacteria
Answer: C
Clarification: Saturation mutagenesis has been used for many years to identify mutations affecting specific crucial biological processes.
7. Insertional mutagenesis facilitates __________
A. Cloning
B. PCR
A. Recombination
D. Centrifugation
Answer: C
Clarification: Insertional mutagenesis leaves a DNA tag in the interrupted gene, which facilitates cloning and gene identification. It is the most popular mutagenesis strategy.
8. Ribozymes are _________ molecules.
A. Toxic
B. Large
A. DNA
D. Catalytic
Answer: D
Clarification: Ribozymes are catalytic molecules that destroy targeted MRNAs. They carry out site-specific cleavage and ligation reactions.