Biochemistry Multiple Choice Questions on “From Genes to Genomes-1”.
1. cDNA, a term used in recombinant DNA technology means ____________
A. Competitive DNA
B. Chemical DNA
C. Complex DNA
D. Complementary DNA
Answer: D
Clarification: Complementary DNA is also termed as cDNA.
2. The process of introduction of foreign DNA into an animal cell is called ____________
A. Transversion
B. Conversion
C. Inversion
D. Transfection
Answer: D
Clarification: Transfection is the deliberate inducing of foreign DNA into an animal cell.
3. A segment of DNA that reads from the same forward and backward is called ____________
A. Palindromic DNA
B. Complementary DNA
C. Plasmid DNA
D. Copy DNA
Answer: A
Clarification: A palindromic sequence is the same when read from 5’ to 3’ on one strand and 3’ to 5’ on its complementary strand.
4. Which of the following enzyme is used in PCR?
A. Taq DNA polymerase
B. HRP
C. EcoRI
D. EcoRII
Answer: A
Clarification: HRP is an enzyme used in ELISA. EcorI and EcorII are the restriction enzymes.
So only a DNA polymerase is responsible for producing multiple copies of DNA sequence.
5. Which of the following is helpful in distinguishing DNA of one individual from another?
A. PCR
B. Reverse transcriptase
C. cDNA
D. RFLP
Answer: D
Clarification: Restriction fragment length polymorphism (RFLP) is helpful in distinguishing one individual from another.
6. Which of the following is the correct order of organization of genetic material from largest to smallest?
A. Genome, chromosome, gene, nucleotide
B. Nucleotide, gene, chromosome, genome
C. Gene, nucleotide, chromosome, genome
D. Chromosome, genome, nucleotide, gene
Answer: A
Clarification: Group of nucleotides makes up a gene, these genes make up a chromosome and these chromosomes make up to genome.
7. Which of the following codons is not a termination codon for protein synthesis?
A. UUU
B. UAG
C. UAA
D. UGA
Answer: A
Clarification: After the ribosome reaches the codon UAG or UAA or UGA on m-RNA strand, protein synthesis is terminated.
8. Which of the following are the two methods of screening?
A. Hybridization and PCR
B. ELISA and blotting
C. ELISA and PCR
D. PCR and RFLP
Answer: A
Clarification: Hybridization and PCR are the two methods of screening.
9. Short sub-sequence of a cDNA sequence is ____________
A. Expressed sequence tag
B. Sequence tagged site
C. Contig
D. YAC
Answer: A
Clarification: Sequence tagged site is a short DNA sequence having single occurrence in genome.
Cotig is a set of overlapping DNA segments that together represent a consensus region of DNA.
YAC is a linear DNA molecule.
10. Short DNA sequence having single occurrence in genome is ____________
A. Expressed sequence tag
B. Sequence tagged site
C. Contig
D. YAC
Answer: B
Clarification: Expressed sequence tag is a short sub-sequence of a cDNA sequence.
Cotig is a set of overlapping DNA segments that together represent a consensus region of DNA.
YAC is a linear DNA molecule.
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