Tissue Engineering Questions and Answers for Entrance exams focuses on “Bioreactors for Musculoskeleton Tissue Engineering”.
1. Sun et al. successfully used an RWV system to establish an in vitro tooth germ culture model.
A. TRUE
B. FALSE
Answer: A
Clarification: Sun et al. effectively utilized an RWV framework to build up an in vitro tooth germ culture model. They saw that tooth germs kept up their commonplace spatial shape both during and after the way of life. Following 6 days of culture, the thick layers of polish and dentin developed on the refined tooth.
2. In traditional ________ bioreactors, cell-laden scaffolds float in the medium while the vessel wall rotates.
A. rotating wall vessel
B. perfusion
C. photo
D. aerobic
Answer: A
Clarification: In conventional RWV bioreactors, cell-loaded frameworks skim in the medium while the vessel diver pivots. An option bioreactor is the turning bed framework (RBS). In this framework, the platforms are fixed on a pole that turns in a half-filled culture vessel.
3. _________ spectroscopy depends on the association of an outse field with the electric dipole moment of the specimen, generally indicated by the permittivity.
A. Dielectric
B. Capacitor
C. Transducer
D. Electrochemical
Answer: A
Clarification: Dielectric spectroscopy estimates the dielectric properties of a medium as an element of recurrence. It depends on the association of an outse field with the electric dipole moment of the specimen, generally indicated by the permittivity. It is likewise a test strategy for portraying electrochemical frameworks.
4. Rotating Wall vessel bioreactors are wely used for Cartilage Tissue Engineering because they prove a favorable Hydrodynamic environment for cartilage differentiation.
A. TRUE
B. FALSE
Answer: A
Clarification: RWV bioreactors have been broadly utilized for ligament TE since they give a good hydrodynamic condition to ligament separation and skeletal muscle tissue recovery. A cell suspension of separated chondrocytes was seeded in an RWV with no platform support. Following 90 days, the scientists got a 3D ligament-like tissue typified by sinewy tissue looking like the perichondrium.
5. For cartilage TE, spinner-flask bioreactors have been used to recreate the in vivo physicochemical conditions of cartilage development.
A. TRUE
B. FALSE
Answer: A
Clarification: For cartilage TE, spinner-carafe bioreactors have been utilized to reproduce the in vivo physicochemical states of ligament advancement. For instance, Liu et al. created the trachea ligament unite in vitro utilizing hare MSCs and a poly(lactic-co-glycolic corrosive) (PLGA. platform in a spinner-flagon bioreactor.
6. Osteogenesis is higher when using spinner-flask bioreactors compared to when using Rotating Wall Vessel bioreactors.
A. TRUE
B. FALSE
Answer: A
Clarification: Osteogenesis is higher when utilizing spinner-flagon bioreactors contrasted with when utilizing RWVs. Rodent MSCs were developed on biodegradable platforms under static and dynamic (spinner flagon and RWV) conditions. Following 21 days of culture, the creators watched higher basic phosphatase (ALP) action and osteocalcin discharge with the spinner-flagon bioreactor contrasted with RWV.
7. Most studies on perfusion bioreactors have focused on the construction of small-sized, engineered bone tissues.
A. TRUE
B. FALSE
Answer: A
Clarification: Most investigations on perfusion bioreactors have concentrated on the development of little measured, designed bone tissues. Creature models were additionally created to look at bone tissue fix [258–260] on the grounds that the bone organization and physiology of pig, goat, sheep, and pooch are like those of people. For enormous scale generation of bone tissues, the generally utilized bioreactors have a few restrictions; in any case, there are some perfusion frameworks that empower refined huge size tissue develops.
Engineering for Entrance exams,