Gene Manipulation Multiple Choice Questions on “Gene Inactivation and Inhibition”.
1. Many gene inactivation strategies don’t require gene modification.
A. True
B. False
Answer: A
Clarification: Many strategies for gene inactivation do not require the direct modification of the target gene. Traditional gene transfer strategies add new genetic information to the genome.
2. Antisense RNA blocks the activity of _______
A. DNA
B. mRNA
A. CDNA
D. RNA
Answer: B
Clarification: Antisense RNA blocks the activity of mRNA in a stoichiometric manner. Antisense RNA has the opposite sense to mRNA.
3. Presence of antisense and complementary sense RNA can lead to the formation of _______
A. Mutation
B. Duplex
A. Carcinogens
D. Protein
Answer: B
Clarification: The presence of complementary sense and antisense RNA molecules in the same cells can lead to the formation of a stable duplex.
4. Antisense RNA can be used in eukaryotes.
A. True
B. False
Answer: B
Clarification: Antisense RNA is used as a natural mechanism to regulate gene expression in a number of prokaryote systems and to a lesser extent in other organisms.
5. Which type of inhibition can be achieved using antisense RNA?
A. Stable
B. Unstable
A. Transient
D. Integrative
Answer: C
Clarification: Transient inhibition of particular genes can be achieved by directly introducing antisense RNA or antisense oligonucleotides into cells.
6. What is MBP?
A. Inducer
B. Protein
A. Promoter
D. Gene
Answer: B
Clarification: An expression cassette was constructed in which the mouse myelin basic protein CDNA was inverted with respect to the promoter.
7. Which gene is targeted in transgenic tomato plant?
A. Mg
B. Pg
A. Gfp
D. Mbp
Answer: B
Clarification: Smith in 1988 generated transgenic tomato plants carrying an antisense construct targeting the endogenous polygalacturonase gene.
8. Level of inhibition does not depend on ________ of antisense RNA.
A. Size
B. Temperature
A. Ph
D. Range
Answer: A
Clarification: The level of inhibition apparently does not depend on the size of the antisense RNA or the part of the endogenous gene to which it is complimentary.