Beer Lambert law is one of the popular topics in analytical chemistry. It relates the weakening of the intensity of the light to the characteristics of the medium through which it is travelling.
Let’s say, we have a clear sample of a drug with a polished surface around its container.
Now, passing electromagnetic radiation (incident radiation or we may use UV rays) to the drug sample, some of the light may get absorbed, and the rest of it gets transmitted.
Here, the intensity of incident radiation = Io, and
The intensity of transmitted radiation = It
So, the absorbance [A=log(frac{I_{o}}{I_{t}})]
∴ It is a unitless quantity.
What will we Study in the Beer Lambert Law?
Here, we will focus on the factors which influence absorption. So, the factors are:
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The concentration of the sample.
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The thickness of the medium.
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The temperature at which we will measure the absorbance.
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The wavelength of the EM radiation.
Point to Remember:
Here, the absorbance of the material will be measured at the wavelength at which we would observe the maximum absorption, and the temperature will be kept at a uniform level.
This is how we will use Beer Lambert law to determine the absorbance of any number of samples. This article will explain Beer Lambert law most simply. So, let’s get started.
Beer Lambert Law Definition
Beer Lambert law consists of the following two laws:
This law is concerned with the thickness of the medium.
This law is concerned with the concentration of the solution via which monochromatic radiation passes.
Lambert’s Law
When monochromatic radiation (it can be UV rays) is passed through a medium, the intensity of the transmitted radiation decreases with the increase in the thickness of the absorbing medium, and it varies directly with the incident radiation.
Mathematically, we can express this statement as:
[-frac{dI_{o}}{dc}]= k’Io (Here, the negative sign indicates the decrease in the intensity of the transmitted radiations)…(1)
Equation (1) says that the rate of decrease in the intensity to the thickness is directly proportional to the incident radiation.
Now, equation (1) can be rewritten as:
It = Io 10-k’b ….(2)
Here, It = Intensity of transmitted radiation
k’ = Proportionality constant
We can write the equation (1) in the following way as well:
[A=log(frac{I_{o}}{I_{t}})]α b
This expression says that the absorbance of light in a homogenous material/medium is directly proportional to the thickness of the material/medium.
So, A = εb….(p)
Beer’s Law
When monochromatic light passes through a ‘transparent medium’, the rate of decrease of transmitted radiation with the increase in the concentration of the medium is directly proportional to the intensity of the incident light.
We can express this statement mathematically as;
[-frac{dI_{o}}{dc}]= kIo ….(3)
We can rewrite this equation as:
It = Io 10-k”c ….(4)
Another form of writing equation (3) is:
[A=log(frac{I_{o}}{I_{t}})] α b
This expression says that the absorbance of light in a homogenous material/medium is directly proportional to the concentration of the sample.
Now, we get our simplified expression as:
A = εb….(q)
Beer Lambert Law Derivation
For determining the Beer Lambert law equation, let’s combine equation (2) & (4), and take the log of these, we get:
[log(frac{I_{o}}{It})]= k’k’’bc….(5)
We can express equation (5) as:
A = εbc…(6)
Equation (6) is the required Beer Lambert Law Formula.
Where,
A = Absorption
ε = Molar absorption coefficient or molar absorptivity in m-1cm-1 = k’ x k’’
b = Thickness of the medium in cm
c = Molar concentration in M
So, the final Beer Lambert law statement is:
When monochromatic radiation passes through a homogeneous medium, then the rate of decrease in the intensity of the transmitted radiation with the increase in the thickness of the medium and the concentration of the solution varies directly with the intensity of incident radiation.
Beer Lambert’s Law Graph
The graphical method is used to determine the value of the Molar Extinction Coefficient (ε). This value is determined by plotting the values of absorbance (on the y-axis) against various values of concentrations (on the x-axis) and determining the slope of the line.
It is observed that a straight line passing through the origin is obtained. We know that,
A=εbc
Where,
A: absorbance of the solution
C: concentration of the solution
ε: Molar extinction coefficient
b: Thickness of the medium
Thus, the slope of the graph is given by εb. We know the value of b, thus we can determine the value of ε easily.
Beer Lambert Law Applications
The important use of the Beer Lambert law is found in electromagnetic spectroscopy.
Now, let’s understand the applications of Beer Lambert law:
Let’s suppose we have a tablet and we don’t know which drug is present in it. Though we may know the drug, then the question arises about what its molar concentration is.
In electromagnetic spectroscopy, we use electromagnetic radiation (we may take UV rays), which scans the tablet and determines the qualitative (drug present) and the quantitative (concentration) property of the tablet.
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The same method can be used in determining the molar absorbance of bilirubin in blood plasma samples.
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We use Beer Lambert Law to conduct a qualitative and quantitative analysis of biological and dosimetric materials that may contain organic or inorganic materials.
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We can determine the concentration of various substances in cell structures by measuring their absorbing spectra in the cell.
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Very often many scientists are given credits for the discovery of this law which are as follows:
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In 1729, Pierre Bouguer discovered this law initially.
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Lambert’s law was proposed by Johann Heinrich Lambert in 1760 after quoting Pierre Bouger’s discovery.
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Beer’s Law was proposed by a scientist named August Beer in 1652.
Fun Facts About Beer-Lambert Law:
Conclusion
Beer Lambert’s Law is a very important topic in Physics as well as in Chemistry. It has a very wide array of applications in many fields. Therefore has brought this article for you.
After reading this article you will get all the information related to Beer Lambert’s Law. to make this topic more interesting for you many applications and fun facts have been added towards the end.
This topic will not only help students preparing for Class 11 and Class 12 examination but also to learners studying in degree and master courses. It can also help technicians and scholars to revise this topic in a very short period.