[Biology Class Notes] on MOET Technology Pdf for EXAM

Multiple Ovulation Embryo Transfer (MOET), the embryo flush, is a common method of breeding cattle. It involves a 5-week superovulation process, which results in the release of several eggs from the donor cow, as opposed to one during natural heat. The cow is implanted and embryos are removed from the uterus the following week. This involves inserting a catheter into the cervix, where the cuff is inflated and fluid comes in and out to collect embryos in each horn.

Drugs used in MOET

Multiple Ovulation Embryo Transfer involves the use of Follicle Stimulating Hormone to promote the release of more than one egg from the ovaries. In addition to Follicle Stimulating Hormones, progesterone (e.g., CIDRs), PMSG (e.g., pregnecol), Gonadotropin-Releasing Hormone (e.g., Receptal), and Prostaglandin (e.g., Estroplan or Estrumate injection) are used to synchronize animals for rotating them at the right time.

What are the Benefits of Multiple Ovulation and Embryo Transfer?

The benefits of MOET are as follows:

• Increased animal fertility.

• No surgery is required.

• The whole process can be easily done on the farm.

• The embryos can be frozen and stored in a laboratory for future use.

• The embryos can be stored in banks for future use.

Collection Goods & Supplies

• Several types of Foley catheters are available; the choice is usually based on the individual’s experience and preferences.

• Different sizes of balls and balloons are available; the choice is determined by the type and size of the female.

• Two types of catheters can be used: two or three methods. Most workers use a two-dimensional catheter with a Y-junction.

• Pre-Warmed Collection Media: Ready-made media for cleaning the embryo are available. Some are complete and ready to use; others may require the addition of a baby calf serum (at least 3 L). Alternatively, Ringer’s lactate may be used. Fetal Filter: A few 0.75 µm filters are available.

Main Goal of Multiple Ovulation and Embryo Transfer

The ultimate goal of raising animals is to improve the productivity of each animal in a very short time at a minimal cost. Genetic development rates are low in developing countries such as India due to some factors such as the unavailability of high-quality genetic material in the required numbers, lack of breeding infrastructure (AI), small herds of farmers, high-cost data recording, and data processing. Veterinary genetic engineering principles form the basis for a variety of selection processes such as interest testing and nucleus breeding programs. These can work in each herd within a group of cooperative farmers. The MOET-ONBS breeding program is a traditional breeding method for livestock development.