Gene Manipulation online test focuses on “Applications of Genetically Modified Mice”.
1. Genetically modified mice can be used to address various aspects of gene function and __________
A. Transcription
B. Regulation
A. Inactivity
D. Temperature gradient
Answer: B
Clarification: Transgenic mice that are mice containing additional transgenes as opposed to those with targeted mutations have been used to address many aspects of gene function and regulation.
2. Many mouse models for human disease have been generated by ______________
A. Transformation
B. Gene-targeting
A. Gene-knockout
D. Conjugation
Answer: C
Clarification: Many mouse models for human diseases have been generated by gene-knockout, but gain-of-function models have also been generated by adding transgenes.
3. Pathology of which of the following disease has been studied using transgenic mouse model?
A. Alzheimer’s
B. Jaundice
A. Prion
D. Tumor
Answer: C
Clarification: Much information concerning the pathology of prion diseases has arisen from the study of transgenic mice expressing mutant prion transgenes.
4. Transgenic mice expressing oncogenes have been used to study ____________
A. Brain tumor
B. Cancer
A. Arthritis
D. Eczema
Answer: B
Clarification: Transgenic mice expressing the oncogenes have been used to study and analyze various aspects and symptoms related to Cancer.
5. When did Brinster construct plasmids MMT gene for constructing transgenic mice?
A. 1941
B. 1961
A. 1981
D. 1951
Answer: C
Clarification: Brinster in 1981 constructed plasmids in which the promoter of the mouse metallothionein-1 gene was fused to the coding region of the HSV Tk gene.
6. Which of the following enzyme is a reporter of the MMT promoter function?
A. Nuclease
B. Thymidine kinase
A. Phosphatase
D. Helicase
Answer: B
Clarification: The thymidine kinase enzyme (TK) enzyme can be assayed readily and provides a convenient reporter of MMT promoter function.
7. The endogenous MMT promoter is inducible by which of the following hormones?
A. Glucocorticoid
B. Insulin
A. Halogenase
D. Amylase
Answer: A
Clarification: The endogenous MMT promoter is inducible by glucocorticoid hormones and heavy metals such as cadmium and zinc, it was then envisaged that hybrid MK gene would be similarly regulated.
8. The MK gene used in the development of transgenic mice was injected into fertilized eggs in the presence or absence of _________ ions.
A. Calcium
B. Cadmium
A. Phosphorus
D. Sulphur
Answer: B
Clarification: The MK gene is injected into the male pronucleus of fertilized eggs, which were then incubated in vitro in the presence or absence of cadmium ions.
9. How many approximate copies of the MK gene were present in transgenic mice expressing the gene?
A. 50
B. 100
A. 150
D. 200
Answer: C
Clarification: The transgenic mice that expressed MK gene was injected with the fusion MK gene into the embryos. There were almost 150 copies of the gene present in the adult.
10. HMG gene is the hybrid between MMT promoter and _______________ hormone.
A. Insulin
B. Cancer
A. Rat death
D. Rat growth
Answer: D
Clarification: In a dramatic series of experiments, Palmiter fused the MMT promoter to the rat growth hormone. This hybrid gene (MGH) was constructed.
11. The phenomena of “position effects” in transgenic mice is related to which of the following?
A. Integrated transgenes
B. Promoters
A. Proteins
D. Inducers
Answer: A
Clarification: In transgenic mice changes in expression of the genes was correlated with changes in methylation of the gene sequences. The complex phenomena of “position effects” affect integrated transgenes.
12. Which of the following vectors are used for the production of transgenic mice?
A. BAC
B. Lambda phage
A. YAC
D. M13 phage
Answer: C
Clarification: The transfer of large DNA segments to the mouse genome has been achieved by transformation with yeast artificial chromosome vectors.
13. Jacobovits (1993) was the first to report the transformation of _____ cells with a YAC vector.
A. Embryonic Stem
B. Liver
A. Pancreatic
D. Brain
Answer: A
Clarification: Jacobovits (1993) were the first to report the transformation of ES cells with a Yeast Artificial Chromosome (YAC. vector via fusion with yeast spheroplasts.
14. Which of the following technique can be used to isolate the vector DNA?
A. Ultracentrifugation
B. Gel-electrophoresis
A. PFGE
D. Electroporation
Answer: C
Clarification: Few alternative strategies involve isolation of the vector DNA by pulsed field gel electrophoresis, followed by an introduction of YAC DNA into mouse cells.
15. YAC transfer to ES cells of mice can be achieved by lipofection.
A. True
B. False
Answer: A
Clarification: The transfer of large DNA segments to the mouse genome has been achieved by transformation with yeast artificial chromosome vectors. But lipofection is a more suitable technique because microinjection involves shear forces that break the DNA.
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