250+ TOP MCQs on Vectors and Cloning in Archae and Answers

Vector Biology Multiple Choice Questions on “Vectors and Cloning in Archae”.

1. Organisms are divided into how many domains?
A. 1
B. 2
A. 3
D. 4
Answer: C
Clarification: Organisms are divided into three domains: Bacteria, Archaea, and Eukarya. Archaebacteria is the other name for the domain Archaea.

2. Eubacteria is the other name for Eukaryotes.
A. True
B. False
Answer: B
Clarification: Organisms are divided into three domains: Bacteria, Archaea, and Eukarya. Eubacteria refers to the domain Bacteria. Eukarya is the domain name for Eukaryotes.

3. What is the minimum number of major groups found in Archae?
A. 1
B. 2
A. 3
D. 4
Answer: C
Clarification: The Archae comprises at least three major groups of prokaryotic organisms with unusual phenotypes when compared to their eubacterial counterparts.

4. The prokaryotic organisms thriving in extreme environments belong to the domain _____________
A. Eukarya
B. Bacteria
A. Archae
D. Eubacteria
Answer: C
Clarification: Archae consists of organisms that are found thriving in extreme environments. These are found usually in high salt concentrations and high temperature regions.

5. Anaerobic methanogens live in rumen of ___________
A. Carnivores
B. Herbivores
A. Scavengers
D. Archae
Answer: B
Clarification: Many of the archaebacteria thrive in extreme environments such as the ones with very high temperatures; others are strictly anaerobic methanogens that live in the rumen of herbivores.

6. Archae exhibit interesting _________ properties.
A. Chemical
B. Physical
A. Physiological
D. Hereditary
Answer: C
Clarification: Archae exhibit interesting physiological properties, coupled with a growing number of complete genome sequences, there has been a considerable interest in developing suitable gene-cloning procedures.

7. Progress of gene cloning in Archae is __________ the Eubacteria.
A. Lagging behind
B. Ahead of
A. Slower
D. Faster
Answer: A
Clarification: All the requirements for gene cloning procedures have been met in Archae, but progress lags far behind that made with major eubacterial group.

8. Which is the only method of getting DNA into the Archae cells?
A. Transformation
B. Transfection
A. Conjugation
D. Electroporation
Answer: D
Clarification: In most Archae investigated so far, electroporation is the only method of getting DNA into cells and the observed efficiencies are low.

9. Efficiency with electroporation in Archae is _________
A. Low
B. High
A. Imponderable
D. Medium
Answer: A
Clarification: Electroporation offers low efficiencies of cloning in archae. But it is the sole techniques investigated so far for organisms belonging to extreme environments.

10. Electroporation efficiency in archae is approximately ______ transformants per cell.
A. 1
B. 10
A. 100
D. 1000
Answer: C
Clarification: Since Electroporation technique of introduction of exogenous DNA in the cells of Archaebacteria offers low efficiency, the transformants generated are mere 100 in number.

11. With polyethylene glycol-mediated transformation _______ efficiencies can be obtained.
A. Lower
B. Higher
A. Medium
D. Very high
Answer: B
Clarification: Much higher frequencies have been observed with some methanogens using polyethylene glycol-mediated transformation or liposome-mediated transformation.

12. With liposome mediated transformation, frequencies of ________ transformants per cell can be obtained.
A. 10⁶
B. 100
A. 1000
D. 10⁷