250+ TOP MCQs on Working with Lipids and Answers

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Biochemistry Multiple Choice Questions on “Working with Lipids”.

1. Which of the following is false about lipids?
A. They are either strongly hydrophobic or amphipathic
B. They are more soluble in water
C. Extraction of lipids from tissues require organic solvents
D. They are insoluble in water
Answer: B
Clarification: Lipids are least soluble in water. They are amphipathic.

2. Which would move faster in thin layer chromatography?
A. Beeswax
B. Phosphatidylinositol
C. Cholesterol
D. Steroid
Answer: A
Clarification: Least polar molecules move faster in thin layer chromatography.

3. In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?
A. High performance liquid chromatography
B. Thin layer chromatography
C. Adsorption chromatography
D. Gas-liquid chromatography
Answer: C
Clarification: During adsorption chromatography, through a column of silica gel solvents of increasing polarity are passed.

4. Phosphatidylinositol, phosphatidylglycerol and phosphatidylserine are easily separated by __________
A. Absorption chromatography
B. TLC
C. HPLC
D. Gas-liquid chromatography
Answer: B
Clarification: TLC can easily separate phosphatidylinositol, phosphatidylglycerol and phosphatidylserine.

5. For the determination of fatty acid composition, transesterification is done in a warm aqueous solution of __________
A. KCl+methanol
B. KOH+methanol
C. NaOH+methanol
D. H2O+methanol
Answer: C
Clarification: In a warm solution of methanol+HCl or methanol+NaOH, transesterification is done.

6. A mixture of fatty acyl methyl esters are separated based on __________
A. Charge
B. Chain length and degree of saturation
C. Molecular weight
D. Ionic size
Answer: B
Clarification: Based on chain length and degree of saturation, mixture of fatty acyl methyl esters is separated.

7. Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?
A. Gas-liquid chromatography
B. Absorption chromatography
C. TLC
D. Centrifugation
Answer: A
Clarification: Gas liquid chromatography or HPLC is preferred in the separation of fatty acyl methyl esters from a mixture.

8. The dye used in TLC for detecting separated lipids by spraying the plate is __________
A. Mordant
B. Alizarin
C. Rhodamine
D. Fuchsin
Answer: C
Clarification: Rhodamine is the dye used in TLC for detecting separated lipids by spraying the plate.

9. Which of the following is not a phospholipase?
A. A
B. C
C. D
D. K
Answer: D
Clarification: A, C and D are phospholipases.

10. In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?
A. Absorption chromatography
B. Thin layer chromatography
C. Gas-liquid chromatography
D. HPLC
Answer: B
Clarification: In TLC, lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones.

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