TRANSCRIPTION Multiple Choice Questions :-
1. Which of the following enzyme is used for synthesis of RNA under the direction of DNA?
A. RNA polymerase
B. DNA ligase
C. DNA polymerase
D. RNA ligase
Answer: A
2. What is the usual sequence of a Pribnow box?
A. AUAUA
B. TATAAT
C. UUUUU
D. TTGACA
Answer: B
3. Which of the following component of RNA polymerase facilitates the recognition of promoter sequences?
A. a subunit
B. b subunit
C. s subunit
D. c subunit
Answer: C
4. Which of the following statement defines a replicon?
A. A DNA molecule that encodes pili for conjugation
B. A DNA molecule that is able to replicate and be maintained
C. A DNA template which is used in transcription
D. The enzyme responsible for transposition
Answer: B
5. Which eukaryotic RNA polymerase makes tRNA’s?
A. RNA polymerase 1
B. RNA polymerase 2
C. RNA polymerase 3
D. Any of these
Answer: C
6. The location of first hexameric sequence typically found in pro-karyotic promoters is
A. at the transcription start site
B. approximately 35 bases upstream of the transcription start site
C. approximately 10 bases upstream of the transcription start site
D. approximately 25 bases upstream of the transcription start site
Answer: B
7. Which of the following is a product of transcription?
A. mRNA
B. tRNA
C. rRNA
D. all of these
Answer: D
8. The sigma factor
A. is responsible for recognizing the termination sequence
B. provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis
C. recognizes the promotor
D. accomplishes DNA restriction
Answer: C
9. The synthesis of mRNA based on a DNA template is called
A. DNA replication
B. transcription
C. translation
D. DNA restriction
Answer: B
10. Recognition/binding site of RNA polymerase is called
A. receptor
B. promoter
C. facilitator
D. terminator
Answer: B
TRANSCRIPTION Objective type Questions with Answers
11. Which of the following enzyme(s) is/are required for lactose fermentation?
A. Transacetylase
B. ß-galactosidase
C. Galactoside permease
D. All of these
Answer: D
12. Which of these catalyzes the synthesis of RNA from a DNA template?
A. DNA gyrase
B. DNA ligase
C. DNA polymerase
D. RNA polymerase
Answer: D
13. A level of regulation, demonstrated by the termination of transcription if tryptophan is abundant, by the tryptophan operon in E.coli cells is called
A. activation
B. attenuation
C. corepression
D. desiccation
Answer: B
14. An mRNA transcript of a gene contains
A. a start codon
B. a stop codon
C. a terminator
D. all of these
Answer: D