300+ TOP Chromatography Objective Questions Answers MCQs

CHROMATOGRAPHY Multiple Choice Questions :-

1. Chromatography is a physical method that is used to separate and analyse __________

a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals

Answer: b

2. In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

a) Column chromatography
b) Planar chromatography
c) Liquid chromatography
d) Gas chromatography

Answer: a

3. In chromatography, the stationary phase can be ___________ supported on a solid.

a) Solid or liquid
b) Liquid or gas
c) Solid only
d) Liquid only

Answer: a

4. In chromatography, which of the following can the mobile phase be made of?

a) Solid or liquid
b) Liquid or gas
c) Gas only
d) Liquid only

Answer: b

5. Which of the following cannot be used as adsorbent in Column adsorption chromatography?

a) Magnesium oxide
b) Silica gel
c) Activated alumina
d) Potassium permanganate

Answer: d

CHROMATOGRAPHY Objective Questions
CHROMATOGRAPHY MCQs

6. Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2mm thick layer of an adsorbent?

a) Gas liquid
b) Column
c) Thin layer
d) Paper

Answer: c

7. Chromatography cannot be used to purify volatile substances.

a) True
b) False

Answer: b

8. In Column chromatography, the stationary phase is made of _________ and the mobile phase is made of _________

a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer: a

9. Chromatography cannot be used to separate delicate products.

a) True
b) False

Answer: b

10. In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________

a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer: a

11. In which of the following type of paper, chromatography does the mobile phase move horizontally over a circular sheet of paper?

a) Ascending paper chromatography
b) Descending paper chromatography
c) Radial paper chromatography
d) Ascending – descending chromatography

Answer: c

12. Liquid chromatography can be performed in which of the following ways?

a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

Answer: c

13. Gas chromatography can be performed in which of the following ways?

a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

Answer: a

14. In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the mobile phase is made of _________

a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer: c

15. Which of the following types of chromatography involves the process, where mobile phase moves through the stationary phase by the influence of gravity or capillary action?

a) Column Chromatography
b) High Pressure Liquid Chromatography
c) Gas Chromatography
d) Planar Chromatography

Answer: d

16. Thin layer chromatography is

A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above

17. In gas chromatography, the basis for separation of the components of the volatile material is the difference in

A. partition coefficients
B. conductivity
C. molecular weight
D. molarity

18. In reverse phase chromatography, the stationary phase is made

A. non-polar
B. polar
C. either non-polar or polar
D. none of these

19. Ion exchange chromatography is based on the

A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography

20. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

A. V = V0 + kDVi
B. V = V0/Vi
C. V = V0 – kDVi
D. V/V0 = kDVi

21. A combination of paper chromatography and electrophoresis involves

A. partition chromatography
B. electrical mobility of the ionic species
C. both (a) and (b)
D. none of these

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