250+ TOP MCQs on Humans Viruses – Reoviridae and Bunyaviridae

Virology online quiz on “Humans Viruses – Reoviridae and Bunyaviridae”.

1. The infectivity of orthoreovirus can be increased by __________
a) increasing the temperature
b) increasing the exposure to proteolytic enzymes
c) increasing the pH
d) increasing the pressure

Answer: b
Clarification: The infectivity of orthoreovirus can be increased by increasing the exposure to the proteolytic enzymes that are usually encountered in the gut. However, they are stable to heat and pH 3.

2. The rotaviruses were discovered in ____________
a) India
b) Australia
c) Africa
d) UK

Answer: b
Clarification: The rotaviruses were discovered in Australia in 1973. It was first discovered in duodenal biopsies, then in feces of children with gastroenteritis in Melbourne. The rotaviruses quickly became the commonest cause of gastroenteritis in infants and are responsible for millions of deaths each year.

3. Which of the following is the common clinical feature of infection caused by rotavirus?
a) Asymptomatic infection
b) Leukemia
c) Pneumonia
d) Bronchitis

Answer: a
Clarification: Asymptomatic infection is the common clinical features of infection caused by the rotavirus. It is common in older children and adults. Sometimes, death may also occur in well-nourished children, a however large number of people die in poorer tropical countries.

4. The capsomers of Orbivirus are ___________
a) hairpin shaped
b) rectangular shaped
c) ring-shaped
d) triangular shaped

Answer: c
Clarification: The capsomers of Orbivirus are ring-shaped or a large doughnut-shaped. The term Orbis means a ring or a circle and hence the virus gets its name as Orbivirus. It contains 10 molecules of double-stranded DNA as its genome.

5. When was the first reovirus isolated?
a) 1944
b) 1951
c) 1955
d) 1961

Answer: b
Clarification: The first reovirus was isolated in the year 1951. It was isolated from the feces of an Australian aboriginal child, by inoculation of infant mice. Reoviruses are one of the three serotypes of mammalian reoviruses belonging to the genus Orthoreovirus.

6. The Puumala virus belongs to the genus _____________
a) Phlebovirus
b) Nairovirus
c) Bunyavirus
d) Hantavirus

Answer: d
Clarification: The Puumala virus belongs to the genus Hantavirus of the family Bunyaviridae. It causes nephropathy in humans and may also develop a hemorrhagic fever with renal syndrome.

7. Which of the following belongs to the Bunyavirus genera?
a) Oropouche
b) Rift Valley fever
c) Seoul
d) Belgrade

Answer: a
Clarification: Oropouche viruses belong to the Bunyavirus genera. The virus mainly causes fever, arthralgia, myalgia, etc. it may also cause a rapid fever illness called Oropouche fever. The rift valley fever virus belongs to Phlebovirus genera whereas Seoul and Belgrade viruses belong to Hantavirus genera.

8. Which of the following cannot detect the growth of Bunyavirus in cultured cells?
a) Immunofluorescence
b) Hemagglutination
c) ELISA
d) PCR

Answer: d
Clarification: The growth of Bunyavirus in cultured cells can be detected by immunofluorescence, hemagglutination, ELISA or Enzyme-Linked Assay (EIA). The isolated virus is assigned to a particular serogroup by complement fixation test.

9. Which of the following is used to screen the population of antibodies?
a) PCR
b) Serology
c) Agarose Gel Electrophoresis
d) Chromatography

Answer: b
Clarification: Serology is often used to screen the population of antibodies and is also used to diagnose illness using paired sera or specific IgM assays. The complement fixation test, EIA, neutralization, and immunofluorescence can also be used for this purpose.

10. The replication of Bunyavirus takes place in mitochondria.
a) True
b) False

Answer: b
Clarification: The replication of Bunyavirus takes place in the cytoplasm. The Bunyavirus enters the host cell through receptor-mediated endocytosis. After the penetration into the host cell, the virion transcriptase is activated and the cascade of events takes place.

11. The Bunyaviridae is a family of RNA viruses.
a) True
b) False

Answer: a
Clarification: The Bunyaviridae is a family of RNA viruses and it mainly contains four genera: Bunyavirus, Phlebovirus, Nairovirus, and Hantavirus. The genome usually consists of three linear segments of single-stranded RNA.